Figure 3 | Mucosal Immunology

Figure 3

From: Primary Heligmosomoides polygyrus bakeri infection induces myeloid-derived suppressor cells that suppress CD4+ Th2 responses and promote chronic infection

Figure 3

F4/80−CD11bhiGr1hi cells increase in the lamina propria (LP) after primary H. polygyrus bakeri infection. Leukocytes were isolated from LP obtained from the small intestines of naive or infected mice on day 7 post infection (p.i.) (n=4 per group). (a) The frequency of CD11bhiGr1hi cells in gated F4/80− cells (top two panels) and CD11b+CD206+ cells in gated F4/80+ cells (bottom two panels) was determined by flow cytometry. (b) Numbers of F4/80−CD11bhiGr1hi myeloid-derived suppressor cells (MDSC) and F4/80+CD11b+CD206+ alternatively activated macrophages (AAMØ)/106 LP leukocytes in naive and day 7 infected mice. ns, not significant; ***P<0.001 (c and d) Diff-Quik stained cytospin preparations of LP leukocytes obtained from naive (c) and infected mice on day 7 p.i. (d) Arrows indicate cells with the characteristic donut-shaped nucleus typical of MDSC. Inset boxes show details of nuclear morphology. Magnification= × 630.

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