Figure 6
Adoptive transfer of CD11c−CD11b+Gr1+ cells from H. polygyrus bakeri-infected mice results in increased egg production and high adult worm burdens in recipient mice. (a) Scheme of the adoptive transfer experiment. Recipient mice adoptively transferred by i.v. injection of 2 × 106 CD4+CD25−CD103− or CD11c−CD11b+Gr1+ cells and control mice injected with 200 μl phosphate-buffered saline (PBS) were infected per os (p.o.) with 200 third-stage larvae (L3) 24 h later. Mice were killed on day 7 post infection (p.i.) for immunophenotyping. Adult worm burdens and egg production were determined in individual mice on days 14 and 28 p.i. (b–d) Bar graphs showing the absolute numbers of CD4+GATA3+ T cells (b), F4/80−CD11bhiGr1hi cells (c), and F4/80+CD206+ cells (d) in the spleens of PBS control mice and recipients of effector CD4+ T cells or CD11c−CD11b+Gr1+ cells. Data are presented as mean±s.e.m. (n=5 mice per group). ns, not significant; *P<0.05; **P<0.01 (e and f) Adult worm burdens and egg counts were determined on days 14 (e) and 28 (f) p.i. Data for individual mice are shown and the mean±s.e.m. of each group is indicated (n=5 mice per group). ns, not significant; *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. Data shown in b–d are representative of three independent experiments. Data shown in (e) are from individual mice pooled from two independent experiments. Data shown in (f) are from individual mice from one experiment.
