Figure 1
Detection of Lactobacillus rhamnosus GG (LGG) in mouse fecal bacteria. Fecal bacteria collected from mice at indicated ages with live LGG (Live-LGG) and glutaraldehyde-fixed LGG (Fix-LGG) treatment from postnatal days 1 to 5 were cultured in deMan, Rogosa and Sharpe broth. DNA was isolated from cultured bacteria for PCR analysis to amplify L. rhamnosus-specific 16S rRNA gene. PCR products were separated on denaturing gradient gel electrophoresis (DGGE) to determine DNA migration profiles (a). Feces of Live-LGG and Fix-LGG treatment were prepared from the same mouse at indicated ages. First lane contains the ladder composed of the PCR product from LGG. LGG colonization was defined as detection of LGG in fecal bacteria. The colonization rate of individual litter was determined by the percentage of mice with LGG colonization in the total number of mice from the same litter. The colonization rate of individual litter detected in 3-week-old mice treated with Live-LGG and Fix-LGG starting at indicated age (postnatal day) for 5 days is shown (b).
