Figure 2
Intestinal CD4 T cells display distinct phenotypic characteristics. B6 mice were orally infected with InlAM rLm and LLO-specific CD4 T cells were analyzed for the indicated markers from the spleen, MLN, LP, and IEL compartment after infection. (a) Representative dot plots with adjunct histograms are gated on LLO-I-Ab+ CD4 T cells at 9 (primary) and 60 (memory) dpi, or 7 days after a secondary challenge of mice that were immunized 60 days previously (secondary). For each dot plot, LLO-I-Ab+ CD4 T cells from the LP (dark blue) are overlaid onto LLO-I-Ab+ CD4 T cells from the spleen (light orange). Numbers within plots are the percentage of cells within gated quadrants and are color coded to the tissue they derive from. (b, e) Representative histograms are gated on live LLO-I-Ab+ CD4 T cells at 9 (primary) and 60 (memory) d.p.i., or 7 days after a secondary challenge of mice that were immunized 60 days previously (secondary). The graphs display pooled data from 9 dpi (primary), between 50 and 90 d.p.i. (memory), and at 7 days after secondary infection of mice immunized 60 days previously (secondary). The graphs show the mean±s.e.m. of pooled data from 2–7 independent experiments with 6–27 mice total. (c, d) LLO-specific CD4 T cells were enumerated from the blood at the indicated days after infection and examined for α4β7, Ly6C, and CD27 expression. (c) Line graph depicts the mean±s.e.m. of three mice per group and representative of two independent experiments. (d) Representative zebra plots from 7 d.p.i. mice are gated on LLO-I-Ab+ CD4 T cells that do or do not express α4β7.
