Figure 5
Pathways/gene groups highlighted by Ingenuity analysis of mRNA data in responders. (a) Ingenuity analysis in interleukin (IL)-13 responders (see Methods and Results for definition) to show top canonical pathways, within 1,214 genes having a false discovery rate (FDR) of <0.05. (b) Schematic to show selected gene expression changes with placebo then nasal allergen challenge (NAC) and curettage at 8 h, compared with baseline gene expression. (c) Effects of a single dose of prednisone (25 mg orally) on gene expression changes with NAC and curettage at 8 h, relative to post placebo NAC at 8 h versus baseline. Genes have been grouped according to type 2 inflammation cytokines (IL4, IL5, and IL13) and receptors (CCR3/eotaxin receptor and IL-3R), a mixed group of inflammatory cytokines (IL1α, IL-1β, IL-2, IL-8, CCL23/MIP-3, OSM, and LTB/TNFC) and receptors (IFNAR1 and IL1R2), complement factors (CFP and C6) and their receptors (C5AR1), and circadian-associated transcription factors and controllers (BMAL-1=ARNTL and CRY1). F2R is thrombin receptor and protease-activated receptor (PAR)-1, and links with the coagulation pathway. Certain complement factors (C1QA, C1QB, C1QC, C1R, and C1S) and circadian-associated transcription regulator genes (CIART (C1orf51), PER1-3, etc) were prominently downregulated by NAC. Red represents upregulation, whereas blue represents downregulation. Blue and red color intensity represents Z-score for the effect.
