Figure 3
From: IL-17A-mediated neutrophil recruitment limits expansion of segmented filamentous bacteria
CXCL1 and CXCL2 are induced by interleukin (IL)-17A and blocking CXCR2 reduces neutrophil influx in response to colonization with segmented filamentous bacteria (SFB)-containing microbiota. (a) Expression of CXCL1 and CXCL2 mRNA were examined by quantitative PCR (qPCR) in the ileum of SFB-void Jax mice colonized with SFB-containing cecal contents (SFB+CC) over the indicated time course. (b) Expression of CXCL1 and CXCL2 mRNA in the ileum of SFB-void Jax mice (Jax) and SFB-void Jax mice treated with isotype control antibody or αIL-17A antibody, and colonized with SFB+CC for 7 days. Data are representative of two independent experiments with four mice per group. (c) Representative fluorescence-activated cell sorting (FACS) plots with associated (d) frequencies and numbers of neutrophils in the ileum of SFB-void Jax mice (Jax) and SFB-void Jax mice treated with isotype control antibody or αCXCR2 antibody, and colonized with SFB+CC for 7 days. Data are representative of four to six mice per group. All data are presented as mean±s.e.m.; **P<0.01 and ***P<0.001, one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test (a) or one-way ANOVA with Tukey’s multiple comparison test (b,d).
