Figure 4
From: IL-17A-mediated neutrophil recruitment limits expansion of segmented filamentous bacteria
Rag-deficient mice colonized with segmented filamentous bacteria (SFB)-containing microbiota fail to induce interleukin (IL)-17A, CXCL1, and CXCL2, and display defective neutrophil recruitment into the ileum. Expression of (a) IL-17A, and (b) CXCL1 and CXCL2 mRNA were examined by quantitative PCR (qPCR) in the ileum of SFB-void Jax B6 and Rag1−/− mice, and SFB-void Jax B6 and Rag1−/− mice colonized with SFB-containing cecal contents (SFB+CC) for 7 days. (c) Representative fluorescence-activated cell sorting (FACS) plots with associated (d) frequencies and numbers of neutrophils isolated from the ileum of SFB-void Jax B6 and Rag1−/− mice, and SFB-void Jax B6 and Rag1−/− mice colonized with SFB+CC for 7 days. Data are representative of two independent experiments with three to four mice per group. All data are presented as mean±s.e.m.; *P<0.05, **P<0.01, and ***P<0.001, one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test.
