Figure 6
From: IL-17A-mediated neutrophil recruitment limits expansion of segmented filamentous bacteria
Neutrophil depletion during colonization with SFB-containing microbiota results in augmented T-helper-17 cells (Th17) responses in the ileum. (a) Expression of interleukin (IL)-17A mRNA in the ileum of segmented filamentous bacteria (SFB)-void Jax mice (Jax) and SFB-void Jax mice treated with isotype control antibody or αLy6G antibody, and colonized with SFB-containing cecal contents (SFB+CC) for 7 days. Data are representative of two independent experiments with four mice per group. (b) Representative fluorescence-activated cell sorting (FACS) plots and cell (c) frequency and number among the indicated groups are shown for expression of IL-17A following restimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin (pre-gated on TCRβ+CD4+ cells). Data are pooled from two independent experiments with four mice per group. All data are presented as mean±s.e.m.; *P<0.05, **P<0.01, and ***P<0.001, one-way analysis of variance (ANOVA) with Tukey’s multiple comparison test.
