Figure 3
Dual oxidase 2 (DUOX2) has a role in hypoxia-induced secretion of high-mobility group box 1 (HMGB1). (a) Gene expression of dual oxidase 1 (DUOX1) and DUOX2 relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in normal human nasal epithelium (NHNE) cells after transfection with shCont, shDUOX1, and shDUOX2. (b) NHNE cells transfected with shCont, shDUOX1, or shDUOX2 were incubated under hypoxic conditions for 8 h and stained with dichlorofluorescin diacetate (DCFDA) for 20 min at 37 °C, to visualize reactive oxygen species (ROS). (c) Mean fluorescence of the images in (b) was quantified using Image J software. Results are presented as mean±s.e.m. (N=3). (d) HMGB1 enzyme-linked immnunosorbent assay (ELISA) was performed to compare the HMGB1 protein levels secreted into extracellular space in the presence of short hairpin RNAs (shRNAs; N=3). *P<0.05 and **P<0.01.
