Figure 3
Increased IL-4 production by TFH cells in PP of eosinophil-deficient mice infected with H. polygyrus. (a, b) Exemplary FACS plots and frequencies of CXCR5 and PD-1 expression by PP CD4+ T cells (a) and mLN (b) CD4+ cells from naive and H. polygyrus-infected WT (black bars) and ΔdblGATA-1 mice (open bars). (c, d) Exemplary FACS plots and frequencies of IL-4+ CD4+CXCR5-PD-1- non-TFH and CD4+CXCR5+PD-1+ TFH cells from PP (c) and mLN (d) stimulated with PMA/ionomycin for 3 h at day 14 p.i. (e, f) IL-4 median fluorescence intensities detected for non-TFH and TFH cells from PP (e) and mLN (f). (g) IL-4+ TFH cells expressed as frequencies of total CD4+ cells in PP. (h) Total number of PP IL-4+ TFH cells. (i, j) Quantification of IL-10+ non-TFH and TFH cells in the PP (i) and mLN (j). Mean±s.e.m. n=4–5. Data are representative of four independent experiments. *P<0.05, ***<0.001, ****P<0.0001. Level of significance comparing naive with infected groups is depicted by+.
