Figure 4
ITLN1 contributes to HDM-induced IL-25, IL-33, and TSLP expression and the activation of EGFR and ERK pathway in human bronchial epithelial cells. (a–d) The kinetics of ITLN1, IL-25, IL-33, and TSLP expression in BEAS-2B cells after exposure to HDM were determined by quantitative PCR. n=4 wells per group. Data are mean±s.e.m. **P<0.01; ***P<0.001 vs. BEAS-2B cells treated with PBS. (e–h) The expression of ITLN1, IL-25, IL-33, and TSLP in BEAS-2B cells transfected with shRNA against ITLN1 or scrambled shRNA and exposed to HDM for 6 h. n=4 wells per group. Data are mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001 vs. BEAS-2B cells treated with ITLN1 shRNA. (i) HDM stimulation increased EGFR and ERK phosphorylation, but ITLN1 shRNA transfection inhibited HDM-induced EGFR and ERK phosphorylation. Densitometry of phospho-EGFR relative to total EGFR (j), and phosphor-ERK relative to total ERK (k). Data are mean±s.e.m from triplicates. ***P<0.001. The data are representative of three independent experiments.
