Figure 5
Itln KD mice are protected from allergic skin inflammation in MC903-induced AD model. (a) Ear thickness was measured daily before the daily application of MC903 or ethanol to the right ear. n=5–8 mice per group. (b) The number of eosinophils in the ear was counted in 10 randomly selected views of one representative ear section from each mouse. (c) Serum total IgE level in peripheral blood was determined by ELISA. n=5–8 mice per group. (d) Itln transcript levels in ear tissue were determined by quantitative PCR. n=5–8 mice per group. (e) H&E staining, Masson trichrome staining, and Itln immunohistochemistry of representative ear section. Original magnification, × 200. Data are mean±s.e.m. **P<0.01; ***P<0.001 vs. Itln KD mice after MC903 exposure. Results are representative of three independent experiments.
