Figure 3
The variation in the pathogenicity of C. albicans isolates did not correlate with their origin. WT mice were infected with C. albicans SC5314, ATCC14053, UC820 or CEC3605. (a) Body weight of infected mice. Data are the mean+s.d. (n=6, pooled from two independent experiments). (b,c) Tongue fungal burden on day 3 and day 7. (d) Quantification of tongue CD11b+ Ly6C+ Ly6G+ neutrophils (day 1) by flow cytometry. (e,f) Il17a and S100a9 expression in the tongue (day 1) was assessed by qRT–PCR. (g) Representative images of sagittal tongue sections (day 1) stained with PAS. Arrows: inflammatory infiltrates. Scale bars: 50 μm. Inset: twofold magnification. (h–j) Cxcl2, Il6, and Tnf expression in the tongue on day 1 was assessed by qRT–PCR. (k) Serum G-CSF on day 1 was quantified by ELISA. (l) Il17f expression in the tongue (day 1) was assessed by qRT–PCR. (m) Scatter plot with data from Figures 1f, 2a and 3d. Color code: mucosal isolates (grey), blood isolates (black). In b–f and h–l, each symbol represents one mouse. Data are the pool of two independent experiments (except for the CEC3605 group in b, which was from one experiment). The mean (a, k) or geometric mean (b–f, h–j, l) of each group and significant differences between each group and the SC5314-infected group are indicated. Solid line in d–f, h–l: (geo)mean of a naive control group. IL6, interleukin-6; Tnf, tumor necrosis factor; G-CSF, granulocyte-colony stimulating factor; qRT–PCR,quantitative reverse transcriptase–PCR; WT, wild type.
