Figure 1
Progressive development and maintenance of HSV-1-induced corneal neovascularization post-virus clearance. Mice were inoculated with HSV-1 onto the killed corneas, and at indicated days post infection (pi) the corneas were collected, stained, and mounted en face on slides for image capture and analysis. (a) Representative images of the corneas with lymphatic (green) and blood (red) vessels at indicated days pi. Each image represents a Z-stacked quadrant of a cornea. White dotted lines demarcate the limbal region from the cornea proper. Bar=200 μm. (b) Metamorph quantification of the corneal area containing lymphatic vessels. (c) Metamorph quantification of the corneal area containing blood vessels. Data represent summary of mean±s.e.m. of two independent experiments with n=10–12 corneas per time point. **P<0.01, ***P<0.001, ****P<0.0001 comparing infected mice to UI control animals as determined by one-way ANOVA followed by Dunnett’s multiple comparisons test. UI, uninfected.
