Figure 2
Leukocytes infiltrate the cornea beyond the resolution of HSV-1 infection. Mice were inoculated with HSV-1 and the corneas were excised at the indicated times post infection. (a) Flow cytometric analysis results of Gr1+F4/80− (neutrophils), Gr1+F4/80+ (inflammatory monocytes), Gr1-F4/80+ (macrophages), CD3+CD4+ T cells, CD3+CD8+ T cells, and NK1.1+CD3− (natural killer, NK) cells are included. (b) Representative Z-stacked 3D still images of a highly vascularized cornea from a day 30 post infected mouse indicating lymphatic vessels (green), blood vessels (red), and CD45+ leukocytes (blue). Each image is a snapshot of a 3D cornea as it rotates clockwise showing endothelium (Endo), stroma (St), and epithelium (Epi). Bars=50 μm. Results indicate summary of mean±s.e.m. of two independent experiments with n=8–10 mice per time point. *P<0.1, **P<0.01, ***P<0.001, ****P<0.0001 comparing infected mice to uninfected (UI) control animals as determined by one-way ANOVA and Dunnett’s multiple comparisons test.
