Figure 2
RvD1 reduces RP73 load and leukocyte infiltration in acute and chronic lung infection. (a, b) Total numbers of live bacteria and inflammatory cells in mice receiving gavage (200 μL of saline) plus vehicle (0.5%vol/vol EtOH/mouse) or RvD1 (100 ng/mouse) 3 h post intratracheal inoculum with planktonic RP73. BAL and lungs were collected at 1 DPI. *P=0.021; **P=0.041; ***P=0.027. Results are mean±s.e.m. from 2 independent experiments with 11 (vehicle-treated group) and 12 (RvD1-treated group) mice. (c–e) Differences in survival, weight loss, and indexes of lung permeability. Mice receiving intratracheal inoculation of RP73-charged agar beads were treated with vehicle (0.05%vol/vol EtOH/mouse, per os) or RvD1 (100 ng/mouse/day per os) via gavage 24 h following inoculum. BAL levels of IL-1β, total proteins, and Evan’s Blue amounts were determined at 5 DPI. *P=0.018; **P=0.002; ***P<0.001. Results are pooled from 2 independent experiments with 12 mice/group. V, vehicle-treated mice; R, RvD1-treated mice. (f, g) Total CFU and leukocytes in vehicle- or RvD1-treated mice infected with agar-embedded RP73 determined 5 days post agar bead inoculum. *P=0.023; ***P<0.001. Results are mean±s.e.m. from 2 experiments with 12 mice/group.
