Figure 2
From: Jak3 deficiency blocks innate lymphoid cell development
Wolverine mice have decreased lymphoid development. (a–h) Spleen was isolated from Nr1d1+/+ WLVRN or unrelated WT control mice. Lymphoid (a-e) and myeloid (f-h) populations were assessed. (a) Gating strategy and representative flow plots for lymphocyte analysis. (b) Frequency and (c) total number of lymphocyte subsets. (d) Gating strategy, representative flow plots, and (e) quantification of T-cell populations. (f) Gating strategy and representative flow plots for myeloid analysis. (g) Frequency and (h) total number of selected myeloid subsets. (i-l) CD45.2 WT or WLVRN donors were transplanted into CD45.1 WT lethally irradiated recipients. Frequency of recovered (i) spleen lymphocytes and (j) spleen myeloid cells from bone marrow chimeras and (k,l) the degree of chimerism per subset 8 weeks post irradiation. (a–l) Data represent n=5 mice per group from two to three independent experiments. *P<0.05, **P<0.01, two-tailed Mann–Whitley test. WT, wild type.
