Figure 1
From: TH17 cells express ST2 and are controlled by the alarmin IL-33 in the small intestine
IL-33 is upregulated in intestinal epithelial cells (IEC) in the proximal part of the small intestine upon CD3-specific antibody injection. C57BL/6 (a–d) or IL17A-eGFPxFoxp3-mRFP reporter (e–f) mice were injected i.p. with anti-CD3 at days 1, 3, and 5. Four hours after each injection, the different parts of the intestine were isolated, as well as the spleen and mesenteric lymph nodes (MLN) (control tissues). (a) Histological analysis of IL-33 (green), EpCAM (red), and PECAM (blue) in the duodenum is shown at steady state and after the first injection of anti-CD3 antibody. (b) Histological analysis of IL-33 (green) and Hoechst (white, nuclear counterstaining) in the spleen and MLN is shown at steady state and after the first injection of anti-CD3 antibody. (c) Quantitative PCR analysis (qPCR) of Il33 mRNA was performed for each tissue at every time point. (d) Immunohistochemistry (IHC) analysis of IL-33 (green), EpCAM (red), and PECAM (blue) in the different parts of the SI in steady state and upon anti-CD3 injection. (e) Duodenal Il33 mRNA was measured by qPCR 4 and 24 h after each injection. (f) IHC of the duodenum at the indicated time points showing the localization of IL-33 (green), IL-17A (red, GFP), CD11c (blue), and Hoechst (white, nuclear counterstaining). Red arrows indicate anti-CD3 injections. qPCR data are presented as mean±s.e.m. and normalized to the values in steady state. Data are representative of ≥3 independent experiments (n=4 animals per group).
