Figure 3
From: TH17 cells express ST2 and are controlled by the alarmin IL-33 in the small intestine
St2 −/− mice show a specific increase of pathogenic TH17 cells in the SI upon anti-CD3 injection. (a) St2 deficient (St2−/−) and wild-type C57BL/6 (wt) mice were injected i.p. with anti-CD3 and T cells were isolated from SI and spleen 4 h after injection to be characterized by flow cytometry. A representative FACS dot plot for each group (n=4) and the associated frequencies of TF-(RORγt and Foxp3) and cytokine-(IL-17A, IFN-γ, TNF-α) expressing T cells isolated from the spleen (b) or the SI (c) are shown. (d) Absolut numbers of CD45+CD4+TCRβ+RORγt+ or IL-17A+ T cells in the SI. Results are shown as mean±s.e.m. and are representative of two independent experiments.* P<0,05, **P<0,01, ***P<0,005 as calculated by unpaired Student’s t-test. ns, not significant. Backgating strategy shown in Supplementary Figure S2a. (e) Mouse naive sorted CD4+CD25−CD62L+CD44− T cells from St2−/− or wt mice were stimulated in vitro with anti-CD3/CD28 and grown under TH17 conditions for 5 days. Expression of Rorc, Il17a, and Ifng in St2−/− TH17 cells was analyzed by qPCR and compared with wt TH17 cells (dotted line). Data are shown as mean±s.d. and are representative of three independent experiments.
