Figure 2
Immune cells in the caecum and ascending colon migrate to exclusively distal part of MLN. (a) Caecum and ascending colon (proximal colon) of KikGR/Foxp3hCD2/hCD52 mice were photoconverted as indicated in the diagram. (b) Expression of KikGR-Green and KikGR-Red by cells isolated from the proximal colon of KikGR/Foxp3hCD2/hCD52 mice before and after photoconversion. (c) KikGR-Green and KikGR-Red expression by cells isolated from dMLN and mMLN 6 h after photoconversion of the proximal colon of KikGR/Foxp3hCD2/hCD52 mice. (d) Proportions of photoconverted cells among CD103+ DCs in dMLN, mMLN, and CLNs (pool of cervical and axillary LNs), 24 h after photoconversion of the proximal colon or small intestine (except for terminal ileum). Flow cytometry gating strategy for CD103+ DCs is shown in Supplementary Figure S4a. Flow cytometry data in b and c are representative of at least two independent experiments with three mice per group; values in the plots indicate the percentage of the parent population. Data in (d) is represented as means±SEM (d; n=5). Statistical comparisons were performed using Mann–Whitney’s U-test (*P<0.05).
