Figure 3
Tregs egress the colon in an S1PR1-dependent manner and migrate to dMLN. Proximal colons of KikGR/Foxp3hCD2/hCD52 mice were photoconverted and flow cytometric analysis was performed. Flow cytometry gating strategy for CD8+ T cells, Tconvs, and Tregs is shown in Supplementary Figure S2b and c. (a) The proportions of photoconverted cells in each T-cell subset at the site of photoconversion immediately after photoconversion. (b) The proportions of photoconverted cells in each T-cell subset in dMLN, mMLN, CLNs (pooled axillary and cervical lymph nodes), and spleen at indicated time points following photoconversion (n=5–6). (c) FTY720 or vehicle was administered 30 min prior to photoconversion of the proximal colon of KikGR/Foxp3hCD2/hCD52 mice and flow cytometric analysis was performed 24 h later. Proportions of photoconverted cells in each subset in the proximal colon (left), and dMLN (right) are shown. Data are representative of at least two independent experiments with three mice per group. Data in graphs represent means±s.e.m. (n=3–5). Statistical comparisons were performed using Mann–Whitney’s U-test (**P<0.01, *P<0.05).
