Figure 4

Colonic migratory Tregs in dMLN express higher levels of immunosuppressive molecules. (a) Proximal colons of KikGR/Foxp3^hCD2/hCD52 mice were photoconverted and flow cytometric analysis was performed 24 h following photoconversion. Proportions of CD44^high CD62L^low cell out of colonic migratory photoconverted Tregs (red) and non-photoconverted Tregs (green) in dMLN, mMLN, and CLNs. Data in graphs are represented as means±SEM (n=3). Statistical comparisons were performed using Mann–Whitney’s U-test (**P<0.01). (b) CD103, ICOS, LAG3, PD-1, and CTLA-4 expression in non-photoconverted LN Tregs and colonic migratory photoconverted Tregs in dMLN (n=4–6). (c) Flow cytometry gating strategy for CD25⁺ and CD25 ^lo/– Tregs in dMLN, and CD103, ICOS, LAG3, PD-1, and CTLA-4 expression in photoconverted and non-photoconverted CD25⁺ and CD25 ^lo/– Treg subsets (n=4–6). (d) Colonic migratory photoconverted Tregs in dMLN, mMLN, and CLNs (n=4–5). Flow cytometry data are representative of at least three independent experiments; values in the plots indicate the percentage of the parent population.

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