Figure 5
Colitis leads to increases in Treg number and proliferation in the colon and dMLN. Timeline for DSS-induced colitis is shown. Mice were given 3% DSS in drinking water for 7 days, then water alone. Relative body weight (a), and Treg number in the whole colon, dMLN, and mMLN in Foxp3hCD2/hCD52 mice following induction of colitis (b). These data are representative of at least 2-3 independent experiments. (c) Proportions of mAzami-Green+ mKusabira-Orange2– cells out of CD8+, Tconv, and Treg subsets in dMLN and whole colon in DSS-treated and control Fucci-Tg/Foxp3hCD2/hCD52 mice on day12 (Colitis). (d) dMLN of KikGR/Foxp3hCD2/hCD52 mice with DSS-induced colitis and control mice were photoconverted on day12 (Colitis) and flow cytometric analysis was performed 24 h later. Flow cytometry data are representative of at least three independent experiments; values in the plots indicate the percentage of the parent population. Data in graphs is represented as means±s.e.m. (n=5–6). Statistical comparisons between day 0 and indicated day in each group were performed using Mann–Whitney’s U-test (**P<0.01, *P<0.05).
