Figure 6
Colitis increases Treg turnover in the colon and Treg migration from the colon to dMLN. Proximal colons of DSS-treated and control of KikGR/Foxp3hCD2/hCD52 mice (Colitis) were photoconverted on day 12 and flow cytometric analysis was performed immediately after photoconversion and 24 h later. The proportions of photoconverted Tregs at the site of photoconversion (proximal colon) (left panel), dMLN (middle panel), and mMLN (right panel) are shown. These flow cytometry data are representative of at least three independent experiments; values in flow cytometry plots indicate the percentage of the parent population, values in parenthesis in bar graph indicate the percentage of photoconverted cells after 24 h out of photoconverted cells at time 0. Data in bar graphs represent means±s.e.m. (n=5–8). Statistical comparisons were performed using Mann–Whitney’s U-test (**P<0.01, *P<0.05).
