Figure 7
Highly proliferative and motile CD25++ Tregs are induced in colitis. (a) Flow cytometry gating strategy for CD25++ and CD25+/– Treg subsets in dMLN and whole colon. (b) Time course of CD25++ Treg cell number in the whole colon, dMLN, and mMLN following induction of DSS-induced colitis. (c) The proportions of mAzami-Green+ mKusabira-Orange2– cells in CD25+/– and CD25++ Tregs in the dMLN and whole colon in DSS-treated and control Fucci-Tg/Foxp3hCD2/hCD52 mice on day 12 (Colitis). (d,e) dMLN (d) or proximal colon (e) of DSS-treated and control KikGR/Foxp3hCD2/hCD52 mice on day 12 (Colitis) were photoconverted and flow cytometric analysis was performed 24 h later. The proportions of photoconverted CD25+/–and CD25++ Tregs in indicated tissues are shown. These flow cytometry data are representative of at least two independent experiments; values in the plots indicate the percentage of the parent population. Data in bar graphs represent means±s.e.m. (n=5–8). Statistical comparisons were performed using Mann–Whitney’s U-test (*p<0.05).
