Figure 1
Electrogenic sodium transport in campylobacteriosis. (a) After mounting the human biopsy specimen into the Ussing chamber, aldosterone was added in a physiological concentration of 3 × 10−9 M to the mucosal and serosal compartments. Eight hours after addition of aldosterone, the epithelial sodium channel (ENaC) blocker amiloride (10−4 M) was added to the mucosal compartment and the resulting drop in ISC was attributed to ENaC-dependent Na+ flux (JNa). Values were corrected for subepithelial resistance contributions in the Ussing chamber with Rt/Repi ratios. *P<0.05, Student’s t-test, n=4. (b) mRNA expression of ENaC subunits. Differential gene expression analysis from RNA-Seq revealed mRNA expression of α-, β-, and γ-ENaC genes (SCNN1 to 3) from unstimulated biopsies. Normalized expression is illustrated as logarithmic function and reflects counts per million (CPM) as calculated by edgeR with *P<0.05, ***P<0.001 vs. controls, n.s., not significant, Fisher’s exact test, n=4 C. jejuni-infected patients, n=6 controls. Data represent mean±s.e.m.
