Figure 2
Epithelial leakage in the colon mucosa of C. jejuni patients. (a) Impedance spectroscopy delineated epithelial (Repi) and subepithelial (Rsub) contributions to the overall transmural electrical resistance (Rt) in human colon biopsies. *P<0.05, Student’s t-test, n=6 each. (b) Epithelial permeability in human colon mucosa for 332 Da fluorescein measured in Ussing chambers. *P<0.05, Student’s t-test, n=6 C. jejuni-infected patients, n=7 controls. (c) Epithelial permeability for 4 kDa FITC-dextran, not significant, Student’s t-test, n=5 C. jejuni-infected patients, n=7 controls. (d) Apoptosis induction. Representative microscopic images (× 400 magnification) from activated caspase-3 (anti-cleaved caspase-3 antibody) immunohistochemistry with red-colored apoptosis induction in crypts of healthy and C. jejuni-infected colon (red arrows in crypt details indicate apoptotic events). White bars=50 μm. (e) Quantification of apoptotic events. Single cells with cleaved caspase-3-positive signals were counted in high power fields (HPF), containing ∼200 epithelial cells or lamina propria lymphocytes. ***P<0.001; Student’s t-test, n=5 each group. Data represent mean±s.e.m.
