Figure 2
Vaccination with alveolar lining fluid (ALF)-exposed Bacillus Calmette-Guérin (BCG) reduces pulmonary inflammation in the Mycobacterium tuberculosis (M.tb) infected lung of vaccinated mice. C57BL/6J mice were vaccinated with NaCl-exposed BCG (NaCl-BCG; gray bars) or ALF-exposed BCG (ALF-BCG; black bars), or left unvaccinated (vehicle; open bars). Six weeks later, mice were infected with a low-dose aerosol of M.tb. (a) At 14 and 250 days post infection (DPI), C57BL/6J mice were killed and lungs fixed, embedded in paraffin, sectioned, and stained with hematoxylin and eosin to visualize tissue morphology. (b and c) Pulmonary inflammation was quantified using Aperio Imagescope (Leica Biosystems, Buffalo Grove, IL). At least 12 distinct lobes were examined from n=3 with 4-5 mice per group per time-point in each experiment. A representative lobe is pictured; pooled data (involvement), mean±s.e.m.; one-way analysis of variance (ANOVA) with Tukey’s post hoc test, *P<0.05 and ***P<0.001; NS: not significant. In all the experiments, for each ‘n’ value, ALFs from different donors were used.
