Figure 3

Vaccination with alveolar lining fluid (ALF)-exposed Bacillus Calmette-Guérin (BCG) preferentially stimulates CD8+ T cells in the lung. C57BL/6J mice were vaccinated with NaCl-exposed BCG (NaCl-BCG; white bars) or ALF-exposed BCG (ALF-BCG; black bars). Six weeks later, lungs were digested and single-cell suspension of cells were labeled with fluorescent antibodies specific for CD8 or CD4 in combination with CD69, CD62L, CCR7, CD44, and intracellular interferon-γ (IFNγ). (a) Total number of CD8+ and CD4+ T cell in the lung. (b) Total number of CD8+ or CD4+ T cells with a memory (CCR7negCD62Lhi CD44hi) phenotype. (c) Total number of CD8+ or CD4+ T cells with an effector (CCR7negCD62Llo CD44hi) phenotype. (d) Total number of CD8+ or CD4+ T cells with the potential to produce IFNγ. (e) Total number of CD8+ or CD4+ T cells expressing CD69. The absolute number of T cells in the lung is shown. Pooled data from n=2 with 4–5 mice per group in each experiment, mean±s.e.m.; Student’s t-test comparing NaCl-BCG vs. ALF-BCG, *P<0.05. Notice that y axis scales among graphs are split different. In all the experiments, for each ‘n’ value, ALFs from different donors were used.