Figure 3
From: MyD88-dependent dendritic and epithelial cell crosstalk orchestrates immune responses to allergens
Immune gene expression in whole lung and ECs at 2 h post-sensitization. (a) BGA plot of immune gene expression in whole lung at 0 and 2 h after OVA/FLA-mediated sensitization of the indicated mouse strains. (b) Venn diagram showing the number of genes differentially expressed (⩾1.5 fold; P⩽0.01, MTI⩾32 for at least one group) between lungs of WT mice (reference group) and the indicated mouse strains at 0 (top) and 2 h (bottom) post-sensitization. (c) Gating strategy for purification of ECs. (d) BGA plot of immune gene expression in sorted ECs of the indicated mouse strains at 2 h post-sensitization. (e) Venn diagrams showing the number of genes differentially expressed between sorted ECs of WT mice and the indicated strains (⩾1.5 fold; P⩽0.01, MTI⩾32 for at least one group). (f–g) Expression of genes in whole lung (f) and ECs at 2 h post-sensitization (g). n=3 mice per group. **P<0.01, ****P<0.0001. BGA, Between group analyses; EC, epithelial cells; MTI, minimum threshold intensity for gene detection; WT, wild type. A full color version of this figure is available at the Mucosal Immunology journal online.
