Figure 4
From: MyD88-dependent dendritic and epithelial cell crosstalk orchestrates immune responses to allergens
Immune gene expression in whole lung, cDCs and AMs at 6 h post-sensitization. (a) BGA plot of immune gene expression in whole lung at 0 and 6 h after OVA/FLA-mediated allergic sensitization of the indicated mouse strains. (b) Venn diagram showing the number of genes differentially expressed between lungs of WT mice (reference group) and lungs of the indicated mouse strains at 6 h post-sensitization (⩾1.5 fold; P⩽0.01, MTI⩾32 for at least one group). (c) Time course of lung gene expression in the indicated strains. **P⩽0.01 as compared to WT reference strain. (d) Gating strategy for purification of AMs and cDCs. (e,f) BGA plot of immune gene expression in cDCs (e) and AMs (f) sorted from lungs of the indicated mouse strains 6 h after OVA/FLA-mediated sensitization. (g,h) Venn diagrams showing the number of immune genes differentially expressed (⩾1.5 fold; P⩽0.01, MTI⩾32 for at least one group) in cDCs (g) and AMs (h) of the indicated strains of mice compared to reference WT mice. (i,j) Relative expression of the indicated genes in purified cDCs (i) and AMs (j) of the indicated mouse strains at 6 h post-sensitization. For purified AMs and cDCs, lungs from three mice were pooled prior to sorting each cell sample. n=3–4 (cDCs) and n=2 (AMs). **P<0.01, ***P<0.001, ****P<0.0001. AM, alveolar macrophage; BGA, Between group analyses; cDC, conventional dendritic cells; FLA, flagellin; OVA, ovalbumin; WT, wild type. A full color version of this figure is available at the Mucosal Immunology journal online.
