Figure 7
From: MyD88-dependent dendritic and epithelial cell crosstalk orchestrates immune responses to allergens
Differential control of Th2 and Th17 cytokine production by EC and DC expression of Myd88 (a,b) Cytokines in mLNs after a single sensitization (a) or two sensitizations (b) with 100 μg OVA and 1.25 μg FLA. (c) T-cell proliferation and cytokine production in co-cultures of OT-II T cells mixed with the indicated APC(s) sorted from lungs of mice 6 h after sensitization with OVA/FLA. (d) Intracellular staining for IL-17 and type 2 cytokines in CD4+ T cells of mice sensitized with OVA/FLA o.p. on days 0 and 7 and collected on day 10. Lung cells were stimulated with Phorbol 12-myristate 13-acetate (PMA) and ionomycin prior to staining. Data shown are (a) representative of two experiments with 8–10 mice per group; (b) from a single experiment, representative of two, 4–5 mice per group; (c) representative of a single experiment performed with duplicate samples; and (d) from a single experiment with 3–4 mice per genotype. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. APC, antigen presenting cell; DC, dendritic cells; EC, epithelial cells; FLA, flagellin; IL, interleukin; mLN, mediastinal lymph node; o.p., oropharyngeal; OVA, ovalbumin.
