Figure 1
Systemic administration of apigenin protects mice from colitis. Single-housed wild-type mice (WTapigenin in blue) were treated with apigenin at days 7 and 3 before induction of colitis by 2% dextran sulfate sodium (DSS; w/v). (a) The body weight loss was daily monitored in sh-WTapigenin and compared with the one of five untreated wild-type C57BL6/J mice (sh-WTuntreated in black). At day 10, the (b) colon lengths, (c) interleukin-1β (Il1b) levels in colonic explants, (d) histological scores, and (e) representative hematoxylin and eosin (H&E)-stained colonic sections are depicted. Error bars show s.e.m. Statistical significance was determined by using either two-way analysis of variance (ANOVA) with Bonferroni corrections for body weight loss or Mann–Whitney test as follows: *P≤0.05, **P≤0.01 and ***P≤0.001. Scale bar=100 μm. Results are representative of three independent experiments.
