Figure 5
The anti-inflammatory properties of apigenin depends on NOD-like receptor family pyrin domain containing 6 (Nlrp6) signaling independently of inflammasome activation in a preclinical model of acute colitis. Single-housed Nlrp6−/− and Pycard−/− mice were treated with apigenin at days 7 and 3 before induction of colitis by 2% dextran sulfate sodium (DSS; w/v). (a) Body weight loss, (b) colon lengths, (c) representative hematoxylin and eosin (H&E)-stained colonic sections, and (d) histological scores are presented for Nlrp6−/− mice (Nlrp6−/−untreated N=4, Nlrp6−/−apigenin N=4). (e) Body weight loss, (f) colon lengths, (g) representative H&E-stained colonic sections. and (h) histological scores are depicted for Pycard−/− mice (Pycard−/−untreated N=4, Pycard−/−apigenin N=5). Error bars show s.e.m. *P≤0.05 and ***P≤0.001. Scale bar=100 μm.
