Figure 4
From: The cytosolic sensor STING is required for intestinal homeostasis and control of inflammation
Frequency of regulatory T (Treg) cells in colon lamina propria (cLP) and mesenteric lymph nodes (MLN) of wild-type (WT) and STING−/− mice. (a) Representative plots of gating strategy for Treg CD4+CD25+Foxp3+ and Treg CD4+LAP+ cells. (b) Frequency of Treg CD4+CD25+Foxp3+ in cLP and (c) MLN of WT and STING−/− mice. (d) Frequency of Treg CD4+LAP+ in cLP and (e) MLN of WT and STING−/− mice. (f) Levels of interleukin (IL)-10 and (h) transforming growth factor (TGF)-β cytokines in colon extract of WT and STING−/− mice. (g) Levels of IL-10 and (i) TGF-β in MLN culture of cells stimulated with anti-CD3 and anti-CD28 for 48 and 72 h, respectively. (j) CD4+CD62L+CD44low or CD4+CD25+ cells were purified by fluorescence-activated cell sorting from the spleen of 8-week WT and STING−/− mice and co-cultured with antigen-presenting cells (APCs) and anti-CD3 stimuli for 3 days at different proportions. Data represent two independent experiments with three to six mice per group. Data represent the mean±s.e.m. #P<0.05; ##P<0.01. CFSE, carboxyfluorescein succinimidyl ester; FSC, forward scatter; LAP, latency-associated peptide; SSC, side scatter.
