Figure 4

(a) Immunostaining for D2-40 in a common melanocytic nevus highlights rare lymphatic vessels (asterisks) in the upper portions (original magnification × 10; scale bar, 100 μm); (b) perilesional D2-40+ lymphatics in a congenital nevus (original magnification × 20; scale bar, 50 μm); (c) intralesional D2-40+ lymphatics in congenital nevus (original magnification × 20; scale bar, 50 μm); (d) D2-40+ lymphatics in peritumoral location in a RGP melanoma with prominent regression (original magnification × 10; scale bar, 100 μm); (e) D2-40+ intratumoral lymphatics showing small lumina compressed by melanoma cells in a VGP melanoma (original magnification × 20; scale bar, 50 μm); (f) D2-40+ peritumoral lymphatics showing large open lumina (original magnification × 40; scale bar, 25 μm); (g) tumor invasive front at the periphery of a thick VGP melanomas shows D2-40+ peritumoral vessels (original magnification × 10; scale bar, 100 μm); (h) large, peritumoral lymphatic vessels at the periphery of a thick VGP melanoma; adjacent blood vessels replete with erythrocytes are negative (original magnification × 20; scale bar, 50 μm); (i) melanoma cells within a lymphatic vessel at the periphery of a VGP melanoma (original magnification × 40; scale bar, 25 μm).