Figure 4

Hierarchical clustering based on the pattern of tumor-infiltrating immune cells. (a) All the ovarian cancer samples, except two, were classified into three main tumor clusters; cluster 1, cluster 2, and cluster 3. The intraepithelial and stromal immune cells formed a cluster with their counterparts. The upper heat map represents normalized numbers of infiltrating immune cells. Individual data for the clinicopathological factors are arranged in the same order as the clustering figure. Histological subtypes are shown by the five colors. The other factors are shown in red and black. Tumor status: pT3 or not, residual tumor: residual tumor positive or negative at the initial surgery, LN meta: lymph node metastasis positive or negative, distant meta: distant metastasis positive or negative. These clinicopathological factors did not deviate among the three clusters. (b) Raw data for the infiltrating numbers of CD8+, CD57+, CD1a+ cells in the three tumor clusters; E, intraepithelial spaces and S, stromal spaces; ^*P<0.05, ^**P<0.01, and ^***P<0.001. (c) Survival analysis of the three clusters. Kaplan–Meier curves for the clusters are shown with regard to overall (left), and progression-free survival (right). The three tumor clusters were different for both overall and progression-free survival (P<0.05, respectively). When individual curves were compared, clusters 1 and 2 were significantly different for both overall and progression-free survival (P<0.05, respectively). (d) COX expression arranged in the same order as the clustering figure. (e) COX-1, COX-2, and COX sum are significantly higher in cluster 1 than cluster 2 by a Mann–Whitney U-test; ^*P<0.05.