Figure 1
From: Hypomethylation of long interspersed nuclear element-1 in hepatocellular carcinomas
Quantification of LINE-1 methylation levels by pyrosequencing of bisulfite-treated DNA samples. The top panel shows methylation of Hep3b cell line, the middle panel of a nonneoplastic liver parenchyma, and the lower panel from the corresponding hepatocellular carcinoma. The PCR product was purified and methylation was quantified using the PSQ HS 96 Pyrosequencing System (Pyrosequencing Inc.). The program quantifies C or G nucleotides for methylated and A or T for unmethylated or mutated sequences. The shaded regions represent CpG sites used for quantification of methylation at each site, and the percent methylation at each site is shown above the peaks.
