Figure 4

Control group. (CD31 and CD34 immunohistochemistry, scale bar=100 μm; c-kit and Ki67 immunohistochemistry, scale bar=50 μm). CD31: normal vascular density in the dermis reflecting the absence of spontaneous angiogenesis in this ischemic area adjacent to gangrene (*). CD34: no angiogenesis is observed within the wall of the vein (v). Normal endothelial layer (arrows). c-kit: absence of c-kit expression in the endothelial cells of dermis vessels adjacent to an ischemic area. Ki67: Absence of proliferation of the endothelial cells in vessels adjacent to an ischemic area.