Table 1 Antibodies used, antigen retrieval and incubation times used for immunhistochemistry. Forward and reverse primer sequences used for PCR

From: Reduced E-cadherin expression correlates with disease progression in Paget's disease of the vulva but not Paget's disease of the breast

Antigen

Source

Clone

Working dilution a

Antigen retrieval

Incubation time

Primer

E-cadherin

Zymed

36

1:250

Microwave

Pressure cooking

1½ h

TTAGGTTAGAGGGTTATCGCGTTAACTAAAAATTCACCTACCGAC

Plakoglobin

Zymed

15

1:100

Microwave

Pressure cooking

1½ h

GCCTGCCTTCTTCTTGTGTCCTGAAGCTTTAGTGGCCAGG

β-Catenin

Zymed

14

1:50

None

1½ h

GGAGGTCTCCTTGGGACTCACTAGTCGTGGAATGGCACC

  1. aDiluted in PBS.
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