Figure 4
From: ZEB1 expression in type I vs type II endometrial cancers: a marker of aggressive disease
In surgical resection specimens of normal human endometrium, ZEB1 expression is confined to the stromal compartment and E-cadherin to epithelial cells; however, in type II endometrial cancers, ZEB1 can be expressed in tumor cells, and E-cadherin expression is lost. Dual immunofluorescent staining of ZEB1 and E-cadherin was performed on human surgical specimens. (a) Dual staining of normal endometrium: ZEB1 (red) is confined to the stroma, whereas E-cadherin (green) is exclusively epithelial (magnification × 400). (b) A malignant mixed Müllerian tumor (MMMT) immunostained for ZEB1 (red) and E-cadherin (green) demonstrates that when ZEB1 is inappropriately expressed in epithelial cells (white arrows), E-cadherin is lost (black arrows). (c) Additional type II endometrial cancers: an MMMT and a uterine serous carcinoma ( × 400 magnification) immunostained for ZEB1 and E-cadherin. When ZEB1 is inappropriately expressed in epithelial cells (white arrows), E-cadherin is lost (black arrows).
