Figure 5
From: ZEB1 expression in type I vs type II endometrial cancers: a marker of aggressive disease
Forced expression of ZEB1 in Ishikawa cells leads to a reduction in E-cadherin protein and increased migration. (a) Whole cell protein extracts were prepared from Hec50co cells, AN3CA cells, wild-type Ishikawa cells, Ishikawa cells stably transfected with human ZEB1-pCIneo and pEGFP-C2, and Ishikawa cells stably transfected with empty pCIneo vector and pEGFP-C2. An immunoblot of 150 μg of protein from each cell line was probed with antibodies recognizing ZEB1, E-cadherin, and α-tubulin. (b) Wound-healing assay using wild-type Ishikawa, Ishikawa-pCI-neo, and Ishikawa-pCI-neo-ZEB1 and the highly migratory Hec50co cells for comparison. Photographs were taken at × 40 magnification at time 0 and 48 h after wounding.
