Figure 1
From: Clinicopathological significance of the CRTC3–MAML2 fusion transcript in mucoepidermoid carcinoma
Artificial generation of CTCR3–MAML2 fusion cDNA from CRTC3 and MAML2 cDNA. Primer A (40 mer) for CRTC3 cDNA amplification includes 20 mer of the MAML2 sequence on the 3′ side, and primer B (40 mer) for MAML2 cDNA containes 20 mer of the CRTC3 sequence on the 5′ side. After amplification of the CRTC3 gene with primer CRTC3A and primer A and amplification of the MAML2 gene with primer B and MAML2A, both polymerase chain reaction (PCR) products are mixed together, and the fusion gene is amplified by PCR using primers CRTC3A and MAML2A. Arrows indicate breakpoints.
