Table 1 Antibodies and probes used for immunohistochemical staining and in situ hybridization
Antibody or probe | Source | Clone | Dilution |
|---|---|---|---|
Immunohistochemistry | |||
B-cell markers | |||
CD20 | Dako | L26 | 1:2400 |
CD79a | Dako | JCB117 | 1:75 |
PAX5 | Biocare | BC/24 | 1:30 |
Germinal center and non-germinal center markers | |||
CD10 | Novocastra | 56C6 | 1:100 |
BCL6 | Novocastra | LN22 | 1:100 |
MUM-1 | Dako | MUM1p | 1:200 |
CD138 | Dako | MI15 | 1:150 |
BCL2 | Dako | 124 | 1:30 |
T-cell markers | |||
CD2 | Novocastra | AB75 | 1:50 |
CD3 | Novocastra | PS1 | 1:150 |
CD5 | Novocastra | 4C7 | 1:100 |
CD7 | Novocastra | CD7-272 | 1:75 |
CD43 | Biotest | MT1 | 1:600 |
Proliferation and activation markers | |||
Ki67 | Dako | MIB1 | 1:250 |
CD30 | Dako | Ber-H2 | 1:30 |
Viral markers | |||
EBV-LMP1 (EBV latent membrane protein 1) | Dako | Cocktail of CS1-4 | 1:800 |
EBNA-2 (EBV nuclear antigen 2) | Novocastra | PE2 | 1:50 |
Human herpes virus 8 | Novocastra | 12B10 | 1:50 |
In situ hybridization | |||
EBER | Novocastra | Epstein–Barr virus probe (fluorescein conjugated) | NA |
Kappa light chain mRNA | Novocastra | Kappa Probe (fluorescein conjugated) | NA |
Lambda light chain mRNA | Novocastra | Lambda Probe (fluorescein conjugated) | NA |
