Figure 1

Flow cytometric analysis for Vβ repertoire. Analysis was based on CD3+CD4+ T cells, and the percentage of each Vβ subtype in the total gated population is shown as a solid bar. (a) A negative case with CD4+ T cells expressing various Vβ subtypes. (b) A positive case with 92% CD4+ T cells expressing Vβ17. (c) A positive case with >70% CD4+ T cells failed to react to any of the T-cell receptor-Vβ antibodies, as ‘non-reactive.’