Figure 2

Vβ flow cytometric analysis-improving detection sensitivity by gating on T cells with an aberrant immunophenotype. Upper panel: Gating on the total CD4+ T cells, Vβ9 expression (45.1%) was below the cutoff and scored as ‘negative.’ Mid panel: Gating on the CD3dimCD4+ T-cell population, Vβ9 expression (87.5%) was above the cutoff and scored as ‘positive.’ Lower panel: Gating on normal CD3+CD4+ T cells, Vβ9 (7.7%) was within the normal range.