Table 1 The laboratory features of patients with mycosis fungoides/Sézary syndrome analyzed by flow cytometry for T-cell receptor Vβ expression

From: Flow cytometric detection of peripheral blood involvement by mycosis fungoides and Sézary syndrome using T-cell receptor Vβ chain antibodies and its application in blood staging

Patients (N=88)

 

Median age (range)

62 (16–94)

Sex (male/female)

49/39

Median WBC/μl (range)

11 813 (3000–67 000)

Lymphocyte %, median (range)

34% (2–89%)

Absolute count/μl, median (range)

5,111 (196–59 630)

CD3+CD4+ lymphocyte %, median (range)

72% (1–99%)

Absolute count/μl, median (range)

4,160 (12–57 792)

SS cells by morphology (mean, range)

 Cases with ≤5%

56 (0.6%, 0–5%)

 6–19%

15 (13%, 6–18%)

 ≥20%

17 (47%, 20–95%)

Aberrant T-cell phenotype (altered CD2, CD3, CD4, CD5 expression levels)

 Normal

57 (65% of cases)

 Abnormal

31 (35%)

CD4+CD7− cells

 Normal (<40% of lymphocytes)

52 (59%)

 Abnormal (≥40% of lymphocytes)

36 (41%)

CD4+CD26− cells

 Normal (<35% of lymphocytes)

26 (30% of cases)

 Abnormal (≥35% of lymphocytes)

62 (70%)

CD4/CD8 ratio

 <10

49 (56% of cases)

 ≥10

39 (44%)

Flow cytometric Vβ analysis

 Negative

22 (25% of cases)

 Positive

66 (75%)

Single Vβ+

44 (50%)

≥70% Non-reactive

22 (25%)

Molecular clonality analysis

 Positive

28 (70% of tested cases)

 Oligoclonal

4 (10%)

 Negative

8 (20%)

 Not performed

47