Table 2 Correlation of abnormal cells detected by morphology, standard flow cytometric immunophenotyping, flow cytometric Vβ analysis, and T-cell receptor gene rearrangement in blood staging

From: Flow cytometric detection of peripheral blood involvement by mycosis fungoides and Sézary syndrome using T-cell receptor Vβ chain antibodies and its application in blood staging

SS by morphology % Sezary cells (mean, range) Absolute count (mean, range)/μl

Control (N=18) (0.2%, 0–3%; 15, 0–110)

≤5% (N=56) (0.6%, 0–5%; 40, 0–532)

>5% but ≤1000/μl (N=5) (10%, 6–14%; 740, 492–880)

1000/μl (N=27) (35%, 10–95%; 7436, 1115–41 195)

Flow cytometric immunophenotype

 Aberrant CD2, CD3, CD4, CD5

1

17

1

13

 Increased CD4+CD7− (≥40%)

3

17

1

18

 Increased CD4+CD26− (≥35%)

3

32

5

25

 Normal

13

14

0

0

CD4:CD8 ratio (≥10:1)

2 (11% of cases)

16 (28%)

1 (53%)

22 (81%)

Flow cytometric Vβ analysis

 Negative

18

21

1

0

 Positive (single Vβ/non-reactive)

0

35 (26/9)

4 (3/1)

27 (15/12)

TCR gene rearrangement

 Positive

3

16

1

11

 Oligoclonal

3

4

0

0

 Negative

6

7

0

1

 Not performed

6

29

4

15

aFinal blood staging (n=88)

NA

 

B0: 24

B1: 25

B2: 39

 
  1. aTumor cells calculated by flow cytometric Vβ analysis.