Figure 2

Examples of immunohistochemical PAT-staining in perilipin-negative human tumors. Antibodies against adipophilin (a2–c2), and TIP47 (a3–c3) react with small lipid droplets in the cytoplasm of cholangiocarcinoma (a1–a4), adenocarcinoma of the colon (b1–b4), and clear cell renal cell carcinoma (c1–c4), whereas no staining is detected with antibodies against perilipin (a1–c1). The respective H&E stain is shown (a4–c4). Original magnification: × 200.