Figure 6

Perilipin, adipophilin, and TIP47 mRNA and protein expression in hepatocellular carcinoma. (a) rtPCR analysis: in 24 different human hepatocellular carcinomas (lanes 1–24) as well as in one control liver (lane 25), DNA transcripts of perilipin, adipophilin, TIP47, as well as of the transcription factors PPARα and PPARγ were detectable at the calculated molecular weight. ^*In two hepatocellular carcinoma (17 and 19), one additional perilipin band was found below (17) and above (19) the estimated DNA transcripts of perilipin. rtPCR analysis was adjusted with the housekeeping gene actin. (b) Immunoblot analysis following SDS–PAGE of proteins of identical 24 hepatocellular carcinomas (1–24) and control liver (25) as shown in (a). Antibodies against adipophilin and TIP47-detected protein bands of 53 and 47 kDa, respectively, antibodies against perilipin (splice variant A) detected protein bands of about 60 kDa as well as further protein bands between 55 and 70 kDa. Protein amounts were adjusted using Bradford assay (20 μg per lane). The respective Coomassie-blue-stained proteins are shown above. Molecular mass markers are given on the right side.